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Cell Constituents and Enzymology-Biotechnology-BSC-Nagpur-Summer-2019

Bachelor of Science (B.Sc.) Semester-2 Examination
BIO-TECHNOLOGY (Cell Constituents and Enzymology)

Optional Paper—2
Time : 3 Hours
Maximum Marks : 50

N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.

1. Describe in detail classification and nomenclature of carbohydrates. [10M]


What are Homopolysaccharides ? Draw and describe the structure of Starch and Glycogen. [10M]

2. Describe the structure of :
(a) Triglycerides [2½M]

(b) Sphingolipids [2½M]

(c) Saturated and unsaturated fatty acids [2½M]

(d) Draw the flow diagram for classification of lipids. [2½M]


Write short notes on :
(e) Classification of terpenes [5M]

(f) Iodine and saponification value. [5M]


3. Discuss in detail classification and nomenclature of enzymes with example and elaborate on E.C.
number. [10M]


Describe in detail the concept of isoenzymes and multi enzymes with suitable examples. [10M]


4. Derive Michaelis-Menten equation and its transformation into equations for straight lines. [10M]


Write notes on :
(a) Effect of pH on enzyme activity [2½M]

(b) Competitive inhibition [2½M]

(c) Effect of temperature on enzyme activity [2½M]

(d) Coupled enzyme assay.[2½M]


5. Solve any TEN of the following :
(i) What are reducing sugars ? [1M]

(ii) Draw the chemical structure of a-D-glucopyranose. [1M]

(iii) What is meant by heteropolysaccharides ?[1M]

(iv) What are glycerophospholipids ?[1M]

(v) Define acid value.[1M]

(vi) What is isoprene rule ?[1M]

(vii) What is an allosteric site ?[1M]

(viii) Who proposed induced-fit hypothesis ?[1M]

(ix) Define coenzymes.[1M]

(x) What is Km ? [1M]

(xi) What is meant by irreversible inhibition ?[1M]

(xii) NAD+ coenzymes containing enzymes can be assayed at _____ wavelength.[1M]


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