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Cell Constituents and Enzymology-Biotechnology-BSC-Nagpur-Winter-2017

Bachelor of Science (B.Sc.) Semester-2 Examination
BIO-TECHNOLOGY (Cell Constituents and Enzymology)

Optional Paper—2
Time : 3 Hours
Maximum Marks : 50

N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.

1. What are homopolysaccharides ? Describe the structure of starch. [10M]


Describe in detail the structure of Glycosaminoglycans. [10M]

2. Write short notes on :
(a) Simple triglycerides [2½M]

(b) Acid value of fats [2½M]

(c) Lecithins [2½M]

(d) Structure of cholesterol. [2½M]


(e) Describe the classification of lipids. [2½M]

(f) Describe Saponification value of fat. [2½M]

(g) Describe unsaturated fatty acids. [2½M]

(h) Describe the structure of gangliosides. [2½M]

3. (a) What are isoenzymes ? Explain with a suitable example. [5M]

(b) Explain the mechanism of metal ion catalysis. [5M]


(c) Describe the classification of enzymes with suitable example. [5M]

(d) Explain the models of enzyme action. [5M]

4. Explain competitive, uncompetitive and noncompetitive Inhibition in detail. [10M]


Write the various spectrophotometric methods for assay of enzymes. [10M]


5. Solve any TEN of the following :
(i) Draw the structure of fructose. [1M]

(ii) Name the sugar present in milk. [1M]

(iii) What are anomers ? [1M]

(iv) Define iodine value. [1M]

(v) Write the chemical structure of 9,12 octadecaenoic acid. [1M]

(vi) Name any one saturated fatty acid. [1M]

(vii) Define irreversible inhibitors. [1M]

(viii) Define holoenzyme. [1M]

(ix) What are allosteric enzymes ? [1M]

(x) What is Katal ? [1M]

(xi) What is turnover number ? [1M]

(xii) What is specific activity ? [1M]


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