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Microbiology – Biotechnology-BSC-Nagpur-Summer-2018

Bachelor of Science (B.Sc.) Semester—I Examination
BIO-TECHNOLOGY (Microbiology)

Optional Paper—1
Time : 3 Hours
Maximum Marks : 50

N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.

1. What is differential staining ? Describe the principle and procedure of gram staining. [10M]


Describe the principle, construction and applications of transmission electron microscope. [10M]

2. Describe the structure of gram positive bacterial cell wall, in detail. [10M]


Describe the process of endospore formation in bacteria. [10M]

3. Describe in detail the lytic cycle of T4 phage. [10M]


(a) Write a note on viral symmetry. [5M]

(b) Write a note on classification of viruses on the basis of nucleic acid. [5M]

4. (a) What are non synthetic media ? Explain with suitable examples. [5M]

(b) How bacteria can be classified on the basis of nutritional requirement ? [5M]


(c) What are micronutrients and macronutrients ? Give one example each. [5M]

(d) Explain enriched and enrichment media with suitable examples. [5M]

5. Solve any ten :

(i) What is numerical aperture ? [1M]

(ii) Write one example of basic dye. [1M]

(iii) Which oil is used while using oil immersion objective ? [1M]

(iv) Flagella are made of which protein ? [1M]

(v) What is meant by staphylococci ? [1M]

(vi) Define plasmid. [1M]

(vii) Name one group of archea. [1M]

(viii) What is a phage ? [1M]

(ix) Write one example of animal virus. [1M]

(x) What is a differential medium ? [1M]

(xi) What is a source of Agar-Agar ? [1M]

(xii) What is peptone ? [1M]


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