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Microbiology – Biotechnology-BSC-Nagpur-Winter-2017

Bachelor of Science (B.Sc.) Semester-I Examination
BIO-TECHNOLOGY (Microbiology)

Optional Paper—1
Time : 3 Hours
Maximum Marks : 50

N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.

1. Describe contributions of Louis Pasteur in the field of microbiology. [10M]


Describe the following :
(a) Gram staining. [5M]

(b) Acid-fast staining. [5M]

2. Describe the structure of cell wall of Gram positive bacteria. [10M]


Describe the process of endospore formation in bacteria. [10M]

3. (a) Differentiate between bacterial and archaeal cell membranes. [2½M]

(b) Give the brief idea of Bergey’s manual of determinative bacteriology. [2½M]

(c) Explain the helical symmetry of viruses. [2½M]

(d) Briefly describe Lysogeny. [2½M]


(e) Explain the concept of three distinct archaeal groups. [2½M]

(f) Differentiate between bacteria and viruses. [2½M]

(g) Classify the viruses on the basis of nucleic acids. [2½M]

(h) Diagrammatically explain Lytic cycle. [2½M]

4. Describe the basic nutritional requirements of bacteria. [10M]


(a) What are natural media ? Give various ingredients used in natural media and explain any two of
them. [5M]

(b) Describe selective and differential media giving suitable examples. [5M]

5. Solve any ten :

(i) Define numerical aperture. [1M]

(ii) What is the importance of oil in oil imersion objective ? [1M]

(iii) What is mordant ? [1M]

(iv) Give one difference between slime layer and capsule. [1M]

(v) What are plasmids ? [1M]

(vi) Why endospores are resistant to high temperature ? [1M]

(vii) What is bacteriophage ?[1M]

(viii) What is enriched media ? [1M]

(ix) What is the source of agar-agar ? [1M]

(x) What are trace elements ? [1M]

(xi) Give any two general properties of viruses. [1M]

(xii) Give any two general properties of archaea. [1M]

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