Optional Paper—1
Time : 3 Hours
Maximum Marks : 50
N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.
1. Explain with ray diagram, working, principle and applications of phase contrast microscopy. [10M]
OR
Write notes on :—
(a) Controversy over spontaneous generations. [5M]
(b) Concept of immunization. [5M]
2. Describe the principle and technique of Gram staining. [10M]
OR
Give a detailed description of Lytic cycle of a Bacteriophage. [10M]
3. Explain the cell wall structure of Gram negative Bacteria. [10M]
OR
Give a detailed description of endospore structure and its formation. [10M]
4. What is growth curve ? Discuss about various phases of growth curve in detail. [10M]
OR
Explain the classification of microorganisms on the basis of Physical conditions required for growth.
[10M]
5. Solve any TEN of the following :—
(i) Define Numerical aperture. [1M]
(ii) Define Resolving power. [1M]
(iii) In what way EM condensers are different from condensers used in light microscopy ? [1M]
(iv) What is meant by simple staining ? [1M]
(v) Name the stains used in acid fast staining. [1M]
(vi) Name any one endospore forming Bacteria. [1M]
(vii) Define Plasmid. [1M]
(viii) What are fimbrae ? [1M]
(ix) What is a capsule ? [1M]
(x) What is meant by continuous culture ? [1M]
(xi) What are microaerophilic bacteria ? [1M]
(xii) Define Generation Time. [1M]