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Microbial Physiology-Microbiology-BSC-Nagpur-Summer-2019

Bachelor of Science (B.Sc.) Semester—2 Examination
MICROBIOLOGY (Microbial Physiology)

Optional Paper—1
Time : 3 Hours
Maximum Marks : 50

N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.

1. Classify the microorganisms on the basis of nutritional requirements. [10M]


Describe in detail selective, enrichment and enriched medium with suitable examples. [10M]

2. Explain in detail bacterial growth curve. [10M]


(a) Classify the microorganisms on the basis of Oxygen requirement. [5M]

(b) Classify the Micro—organism of the basis of pH requirements. [5M]

3. (a) Explain principle and working of autoclave. [5M]

(b) Explain the use of U.V. rays as means of microbial control. [5M]


(c) Explain membrane filter technique. [5M]

(d) Describe the characteristics of an ideal disinfectant. [5M]

4. (a) Explain oligodynamic effect. [2½M]

(b) In controlling microbial growth why 70% alcohol is more effective ? [2½M]

(c) Write a note on gasseous sterilization. [2½M]

(d) Write in brief on factors influencing antibiotic activity. [2½M]


(e) Give the mechanism of cell injury to cytoplasmic membrane. [2½M]

(f) Explain how quaternary ammonium compounds act as anti-microbial agent. [2½M]

(g) Explain mode of action of chlorine as anti-microbial agent. [2½M]

(h) How surface active agent controls the microbes ? [2½M]

5. Solve any ten questions :

(a) Give the chemical composition of nutrient agar. [1M]

(b) What is diauxic growth ? [1M]

(c) What is an axenic culture ? [1M]

(d) Give any two examples of mesophiles. [1M]

(e) Define Psychrophiles. [1M]

(f) In continuous culture the bacterial cells are maintained in which phase of growth ? [1M]

(g) What is HEPA filter ? [1M]

(h) Define incineration. [1M]

(i) What is the difference between disinfectant and sanitizer ? [1M]

(j) Give any two examples of aldehydes as agents for microbial control. [1M]

(k) Define phenol coefficient. [1M]

(l) Give the example of antibiotic which act on the cell wall of bacteria. [1M]

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