LinkedIn Insight Macromolecules-Biotechnology-BSC-Nagpur-Summer-2019 - Grad Plus


Bachelor of Science (B.Sc.) Semester-I Examination
BIO-TECHNOLOGY (Macromolecules)

Optional Paper—1
Time : 3 Hours
Maximum Marks : 50

N.B. :— (1) All questions are compulsory and carry equal marks.
(2) Draw well labelled diagram wherever necessary.

1. Give a detailed account of Maxam-Gilbert method of DNA sequencing. [10M]


Describe in detail Watson and Crick model of DNA. Add a note on A and Z forms of DNA. [10M]

2. Write short notes on :
(a) Concept of split genes [2½M]

(b) Prokaryotic genes [2½M]

(c) Structure of nucleosome [2½M]

(d) Arrangement of histones in the octamer. [2½M]


(e) Telomere [2½M]

(f) Cot curves [2½M]

(g) C-value and C-value paradox [2½M]

(h) Centromere [2½M]

3. Describe the primary structure of proteins with respect to :

(a) End group analysis [5M]

(b) Cleavage of disulfide bond. [5M]


What are amino acids ? Describe the reactions of amino acids with :
(i) Edman’s reagent
(ii) Ninhydrin. [10M]

4. Describe in detail the a-helical and b-pleated sheet structure of proteins. [10M]


(a) Describe the forces that stabilize the tertiary structure of proteins. [5M]

(b) Describe the titration curve of amino acid. [5M]

5. Answer any ten of the following :

(i) Name the pentose sugar present in RNA. [1M]

(ii) Name one unusual base found in tRNA. [1M]

(iii) Chargaff’s rules are applicable to nucleic acids. (True or False) [1M]

(iv) What are exons ? [1M]

(v) What is the role of linker DNA ? [1M]

(vi) What are spacers ? [1M]

(vii) Name any one acidic amino acid. [1M]

(viii) What is meant by an essential amino acid ? [1M]

(ix) Write the chemical structure of alanine. [1M]

(x) What is meant by denaturation of proteins ? [1M]

(xi) What are domains ? [1M]

(xii) Name the metal ion present in myoglobin. [1M]

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