Question: 138: The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following
(1) (a) Cellulase (b) Protease
(2) (a) Cellulase (b) Chitinase
(3) (a) Chitinase (b) Lipase
(4) (a) Cellulase (b) Lipase
Answer: Option (2)
Explanation:
To isolate genetic material, the cell wall must be broken so that cellular contents can be released.
Plant cell walls are mainly composed of cellulose.
Cellulase enzyme is required to break down cellulose and remove the plant cell wall, allowing isolation of genetic material.
Fungal cell walls are mainly composed of chitin.
Chitinase enzyme is required to digest chitin present in the fungal cell wall.
Protease breaks proteins, and lipase breaks lipids, so they are not primarily involved in cell wall digestion of plants or fungi.
Therefore, enzyme (a) is cellulase for plant cells and enzyme (b) is chitinase for fungal cells.